Mitochondrial DNA (mtDNA) analysis enables forensic laboratories to extract genetic data from small biological samples, found in less than ideal condition. Mitochondria in humans cell contain about 1,000 copies of mtDNA. The ease-of-use of next-generation sequencing (NGS) and Nextera XT enables labs to speed up their workflow, reducing time and labor spent on prep, and generate deeper coverage data compared to Sanger sequencing at 1X coverage/rx.
The updated Illumina mtDNA demonstrated protocols provide a complete mitochondrial DNA solution – from targeted library prep and sequencing to bioinformatics analysis and report generation in BaseSpace – to help investigators draw conclusions in a straightforward and intuitive workflow.
The two new mtDNA apps in BaseSpace allow for variant analysis and easy visualization of mitochondrial sequence data. This workflow can analyze any part of the full circular genome, without any origin dead zone, using quality and coverage thresholds customized by the user (Figure 1).
The BaseSpace team is excited to introduce the BaseSpace Professional tier, which enables access to a host of new features and services, including:
- Multi-user access with Workgroups
- 8 hours of Bioinformatics Professional Services from Illumina
- Expandable storage and compute credits
With BaseSpace Professional we are releasing a new Workgroup feature to enable seamless and efficient data sharing for you and your colleagues. Workgroups enable collaboration on a global scale.
We’ve updated BaseSpace to give it a sleeker, more modern look and to simplify the interface, all without compromising the core functions that you’ve come to rely on.
Our BaseSpace design and development teams focused on making it easier for you to access relevant information and tasks, and harmonized the new design with the visual attributes you see in our instruments, like luminosity, transparency, and vibrancy.
We are very excited to announce the availability of the new BaseSpace iOS mobile app. With this app you can now monitor your BaseSpace runs and analyses more easily than ever before using any compatible mobile iOS device running iOS 8.4 or newer! This includes the iPhone 4S and later, and iPad 2 and later devices.
Align Custom Genomes, Improve Scaffolding for de novo Assemblies, and more with our latest BaseSpace Labs Apps
The BaseSpace Labs team have kept busy in recent months, bringing multiple new apps and improved functionality for existing apps to BaseSpace. Our new and improved tools enable alignment of custom genomes with BWA, improved scaffolding for de novo assemblies, coordinate mapping conversion from one human assembly to another, and more!